Publications of Manfred T. Reetz

Li, A.; Qu, G.; Sun, Z.; Reetz, M. T. Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets. ACS Catalysis 2019, 9, 7769–7778.

Cen, Y.; Singh, W.; Arkin, M.; Moody, T. S.; Huang, M.; Zhou, J.; Wu, Q.; Reetz, M. T. Artificial Cysteine-Lipases with High Activity and Altered Catalytic Mechanism Created by Laboratory Evolution. Nature Communications 2019, 10, 3198.

Li, G.; Dong, Y.; Reetz, M. T. Can Machine Learning Revolutionize Directed Evolution of Selective Enzymes? Advanced Synthesis & Catalysis 2019, 361, 2377–2386.

Xu, J.; Cen, Y.; Singh, W.; Fan, J.; Wu, L.; Lin, X.; Zhou, J.; Huang, M.; Reetz, M. T.; Wu, Q. Stereodivergent Protein Engineering of a Lipase To Access All Possible Stereoisomers of Chiral Esters with Two Stereocenters. Journal of the American Chemical Society 2019, 141, 7934–7945.

Yu, D.; Wang, J.-bo; Reetz, M. T. Exploiting Designed Oxidase–Peroxygenase Mutual Benefit System for Asymmetric Cascade Reactions. Journal of the American Chemical Society 2019, 141, 5655–5658.

Dong, Y.; Yao, P.; Cui, Y.; Wu, Q.; Zhu, D.; Li, G.; Reetz, M. T. Manipulating the Stereoselectivity of a Thermostable Alcohol Dehydrogenase by Directed Evolution for Efficient Asymmetric Synthesis of Arylpropanols. Biological Chemistry 2019, 400, 313–321.

Reetz, M. T. Directed Evolution of Artificial Metalloenzymes: A Universal Means to Tune the Selectivity of Transition Metal Catalysts? Accounts of Chemical Research 2019, 52, 336–344.

Sun, Z.; Liu, Q.; Qu, G.; Feng, Y.; Reetz, M. T. Utility of B-Factors in Protein Science: Interpreting Rigidity, Flexibility, and Internal Motion and Engineering Thermostability. Chemical Reviews 2019, 119, 1626–1665.

Zhou, H.; Wang, B.; Wang, F.; Yu, X.; Ma, L.; Li, A.; Reetz, M. T. Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase. Angewandte Chemie International Edition 2019, 58, 764–768.

Acevedo-Rocha, C. G.; Sun, Z.; Reetz, M. T. Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique. Chembiochem 2018, 19, 2542–2544.

Cadet, F.; Fontaine, N.; Li, G.; Sanchis, J.; Chong, M. N. F.; Pandjaitan, R.; Vetrivel, I.; Offmann, B.; Reetz, M. T. A Machine Learning Approach for Reliable Prediction of Amino Acid Interactions and its Application in the Directed Evolution of Enantioselective Enzymes. Scientific Reports 2018, 8, 16757.

Li, A.; Sun, Z.; Reetz, M. T. Solid‐Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution? Chembiochem 2018, 19, 2023–2032.

She, W.; Ni, J.; Shui, K.; Wang, F.; He, R.; Xue, J.; Reetz, M. T.; Li, A.; Ma, L. Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System. ACS Synthetic Biology 2018, 7, 2236–2244.

Li, G.; Garcia-Borràs, M.; Fürst, M. J. L. J.; Ilie, A.; Fraaije, M. W.; Houk, K. N.; Reetz, M. T. Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution. Journal of the American Chemical Society 2018, 140, 10464–10472.

Ilie, A.; Harms, K.; Reetz, M. T. P450-Catalyzed Regio- and Stereoselective Oxidative Hydroxylation of 6-Iodotetralone: Preparative-Scale Synthesis of a Key Intermediate for Pd-Catalyzed Transformations. The Journal of Organic Chemistry 2018, 83, 7504–7508.

König, G.; Reetz, M. T.; Thiel, W. 1-Butanol as a Solvent for Efficient Extraction of Polar Compounds from Aqueous Medium: Theoretical and Practical Aspects. The Journal of Physical Chemistry B 2018, 122, 6975–6988.

Schrimpf, A.; Hempel, F.; Li, A.; Linne, U.; Maier, U. G.; Reetz, M. T.; Geyer, A. Hinge-Type Dimerization of Proteins by a Tetracysteine Peptide of High Pairing Specificity. Biochemistry 2018, 57, 3658–3664.

Li, A.; Acevedo-Rocha, C. G.; Reetz, M. T. Boosting the Efficiency of Site-Saturation Mutagenesis for a Difficult-to-Randomize Gene by a Two-Step PCR Strategy. Applied Microbiology and Biotechnology 2018, 102, 6095–6103.

Acevedo-Rocha, C. G.; Gamble, C. G.; Lonsdale, R.; Li, A.; Nett, N.; Hoebenreich, S.; Lingnau, J. B.; Wirtz, C.; Fares, C.; Hinrichs, H.; Deege, A.; Mulholland, A. J.; Nov, Y.; Leys, D.; McLean, K. J.; Munro, A. W.; Reetz, M. T. P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping. ACS Catalysis 2018, 8, 3395–3410.

Li, G.; Wang, J.; Reetz, M. T. Biocatalysts for the Pharmaceutical Industry Created by Structure-Guided Directed Evolution of Stereoselective Enzymes. Bioorganic & Medicinal Chemistry 2018, 26, 1241–1251.