Chromatography & Electrophoresis

As the central laboratory for separation sciences of the Max-Planck-Institut für Kohlenforschung the Department of Chromatography & Electrophoresis offers an extensive range of analytical methods. We apply instrumental chromatographic and electrophoretic separations as well as hyphenated techniques.

In qualitative analyses we clarify the presence of specific components (i.e. postulated products in a reaction mixture). Over and above that we determine the concentration of requested components in quantitative analyses.

Philipp Schulze

Dr. Philipp Schulze

Vita Publications

since 2010: Head of the Chromatography & Electrophoresis group at the Max-Planck-Institut für Kohlenforschung (Mülheim, Germany)
2009-2010: Head of laboratory at the Max Aicher Group (Freilassing, Germany)
2009: Ph.D. at the University of Regensburg (Germany)
2005: Diploma of the University of Wuppertal
1979: Born in Wuppertal/Germany

2011

12. Microfluidic chips for chirality exploration; S. Nagl, P. Schulze, L. Gitlin, D. Belder; Anal. Chem. 83, 3232–3238 (2011)

2010

11. Rapid determination of neuroactive substances in bananas using microchip electrophoresis with deep UV native fluorescence detection; S. Ohla, P. Schulze, S. Fritzsche, D. Belder; Anal. Bioanal. Chem., DOI 10.1007/s00216-010-4557-z

10. Gas Chemical Studies Using Corona Discharge Reactors; P. Schulze, A. Stankiewicz, M. Aicher, M. Mattner, A. Ulrich; Eur. Phys. J. D. 60, 637-644 (2010)

9. A New Weakly Basic Amino-Reactive Fluorescent Label for Use in Isoelectric - Focusing and Chip Electrophoresis; P. Schulze, M. Link, M. Schulze, S. Thürmann, O.S. Wolfbeis, D. Belder; Electrophoresis 31, 2749-2753 (2010)

2009

8. Rapid replication of master structures by double casting with PDMS; L. Gitlin, P. Schulze, D. Belder; Lab Chip 9, 3000-3002 (2009)

7. Progress in microchip enantioseparations; S. Nagl, P. Schulze, M. Ludwig, D. Belder; Electrophoresis. 30, 2765-2772 (2009)

6. New diode laser-excitable green fluorescent label and its application to detection of bovine serum albumin via microchip electrophoresis; M. Link, P. Schulze, D. Belder, O.S. Wolfbeis; Microchim. Acta 166, 183-188 (2009)

5. Label-free fluorescence detection in capillary and microchip electrophoresis; P. Schulze, D. Belder; Anal. Bioanal. Chem. 393, 515-525 (2009)

2008

4. Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis; P. Schulze, M. Ludwig, D. Belder; Electrophoresis 29, 4894-4899 (2008)

2007

3. Microfluidic glass chips with an integrated nanospray emitter for coupling to a mass spectrometer; P. Hoffmann, U. Häusig, P. Schulze, D. Belder; Angew. Chem. 119, 5000-5003 (2007) Angew. Chem. Int. Ed. 46, 4913-4916 (2007)

2. Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis; P. Schulze, M. Schüttpelz, M. Sauer, D. Belder; Lab Chip 7, 1841-1844 (2007)

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Research Topics

Atmospheric-pressure Plasmas: Atmospheric-pressure Plasmas

The plasma state of matter is defined as a gas wherein a certain portion of particles are ionized. Plasmas can be classified by temperature in thermal and non-thermal plasmas (often referred to as “cold plasmas”). The latter are characterized by a significant disparity of the electron and the ion temperature. Non-thermal plasmas are i.e. used for surface modification, cleaning or coating. We construct non-thermal plasma sources for applications in analytical chemistry.
Instrumental Developments: Instrumental Developments

Our department is engaged in service analytics to support the preparatory chemical research groups in our institute. Therefore, application development as well as instrumental developments in the field of analytical chemistry is performed in close collaboration with our precision engineering workshop.

Instrumentation

Gas Chromatography

Gas Chromatography

For the gas chromatography (GC) there is a great variety of different methods for common separations of chemical compounds with a boiling point ≤ 400 °C at our disposal (i.e. polar, apolar or chiral stationary phases). We provide:

High Temperature GC
Information about constitution, conformation, exact bond lengths and angles as well as the three-dimensional packing in the solid state.
Low Temperature GC
GC x GC
GC – MS

Liquid Chromatography & Electrophoresis

Liquid Chromatography & Electrophoresis

In liquid chromatography (LC) soluble compounds are analysed in a liquid phase. Doing so we carry out predominantly reversed phase and chiral LC separations. The preparatory LC instruments allow the purification of chemical substances in a typical mass range of 1 mg to 10 g. The purified products are analysed by GC, IR, MS, NMR, XPS or are used as educts for chemical synthesis. We provide: